Analysis of Ligation Activity in Miniaturized L1 Ligase Ribozyme Variants Down to 49 nt and in Split Ribozyme Forms
Identifying the smallest active unit capable of ligating RNA in the absence of proteins is important to elucidate ribozyme evolution. Here, we aimed to construct an evolutionary model of the L1 ligase ribozyme. The miniaturized L1 ligase ribozyme (L1X6c2) consists of three stems: Stems A, B, and C. Mutants with shorten...
Yuki Nitta, Misaki Kato, Yunoka Takagi et al.
· International Journal of Mol... · 0 citations