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Zheng Guo

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Review Open access Nov 2026

Integrating noncanonical amino acids and synthetic cofactors for the development of artificial metalloenzymes

Natural metalloenzymes harness the sophisticated synergy between canonical amino acids and metal cofactors to catalyze life-sustaining reactions. However, the limited chemical diversity of the twenty standard amino acids often restricts non-natural transformations. To overcome these constraints, artificial metalloenzym...

Si-Meng Ding, Yu-Xin Shi, Ren-Jun Gao et al. · 0 citations
#protein folding Open access Sep 2026

Consensus design and in silico screening of GH42 β-galactosidase for enhanced catalytic hydrolysis of whey lactose.

This study addresses a major bottleneck in dairy waste management by engineering a highly efficient, thermophilic enzyme for lactose hydrolysis, transforming the cheese manufacturing by-product whey into a high-value resource. While traditional glycoside hydrolase family 2 (GH2) enzymes suffer from poor industrial ther...

Si-Meng Ding, Jia-Jia Li, Jia-Hui Li et al. · 0 citations

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