AiCE-Guided Loop Dynamics Redesign in a Novel Glycosyltransferase Enhances the Dual-Regioselective Glycosylation of Rhein
Abstract Cassia seeds contain bioactive anthraquinones, including rhein and its glycosides, but their efficient production is limited by the low activity and poor regioselectivity of natural enzymes. Here, we identified NtUGT75C1 from Nicotiana tomentosiformis as a glycosyltransferase producing rhein-8-O-β-d-glucoside...