gene editing

pgRNA-m6A levels associate with HBV replication and liver function

Site-specific m6A demethylation of pgRNA1907-m6A significantly reduced HBV DNA, hepatitis B e antigen (HBeAg) and HBV pgRNA, while induced levels of interferon alfa 2 (IFN-α2) and apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) were reduced.

Beyond immune rejection: integrative genetic engineering strategies for overcoming coagulopathy in xenotransplantation

It is argued that scientific progress requires diverse new gene combinations and, ultimately, organ-tailored donor pigs, and the rationale for integrative endothelial humanization is synthesized.

Editorial: Advances in phenomics, metabolomics, and genomics for precision breeding of ornamental horticultural crops

Peony represents one of the world's most valuable ornamental crops, prized for its aesthetic appeal and expanding commercial significance. However, conventional propagation methods remain slow and inefficient, limiting the rapid dissemination of elite cultivars and the implementation of precision breeding strategies. S...

Immune gene and protein profiles across blood, cerebrospinal fluid and peripheral nerve in inflammatory neuropathies

Public aggregate materials accompanying the Human Genomics submission manuscript v42. Version 2.3.0 includes the revised manuscript with five embedded main figures and three editable tables, updated figure labels and legends, the edited graphical abstract, four supplementary figures, a 49 worksheet aggregate data workb...

Enzymatic skeletal editing reaction forming the 1,3,5-triazine core during biosynthesis of the anticancer nucleoside analogue 5-azacytidine

Programmable RNA-Guided DNA Recombination: Mechanisms, Engineering, and Applications

This comparative framework highlights a broader transition from programmable sequence modification toward direct engineering of genomic architecture, while identifying recognition-site constraints, mismatch-tolerant recombination, unintended recombination products, delivery, and genome-wide specificity as key translati...

Immune gene and protein profiles across blood, cerebrospinal fluid and peripheral nerve in inflammatory neuropathies

Public aggregate materials accompanying the Human Genomics submission manuscript v42. Version 2.3.0 includes the revised manuscript with five embedded main figures and three editable tables, updated figure labels and legends, the edited graphical abstract, four supplementary figures, a 49 worksheet aggregate data workb...

Genetically Modified and Gene-Edited Organisms—Objectives, Public Perception and Applications

There is a need to reconsider and redefine the term GMO as well as the acceptance of products created in this way.

Transgenic overexpression of GmAPC7-CT improves seed yield and reduces susceptibility to soybean mosaic virus and Meloidogyne incognita in soybean

Stable transgenic soybean lines overexpressing the GmAPC7-CT gene have demonstrated increased seed yield and reduced susceptibility to the soybean mosaic virus and Meloidogyne incognita. The Anaphase-Promoting Complex subunit 7 (APC7) is a core structural component of the anaphase-promoting complex or cyclosome (APC/C)...

An improved protocol for high-efficiency and cost-effective CRISPR/Cas9-mediated knock-ins in C. elegans

This protocol provides a simplified CRISPR/Cas9-mediated knock-in pipeline suitable for editing a large number of loci, and offers cost-effective, alternative ssDNA donor preparation strategies, overcoming the requirement of expensive locus-specific, long phosphorylated primers.

pgRNA-m6A levels associate with HBV replication and liver function

m6A modification has been shown to play a role in regulating HBV RNA expression. However, it remains unclear whether the m6A levels of HBV pregenomic RNA (pgRNA) are related to HBV replication and liver function. Serum HBV pgRNA-m6A levels were determined by T3 DNA ligase assay. HBV DNA copy numbers and gene expression...

Programmable enzymes for targeted gene insertion.