gene editing

Self-extending DNA recording with stochastic and programmed writing

Recording cellular histories requires new information to accumulate without erasing earlier records. Here we introduce Infinite Printer, a CRISPR recording architecture that couples self-extension to regeneration of a writable terminal context. A self-targeting prime-editing guide RNA (stpegRNA) directs repeat extensio...

Genetic and Epigenetic Manipulation Using CRISPR Techniques in Ewing Sarcoma

Translating base editors: a decade of progress from the bench to the clinic

Consumers’ Perception of Genetically Biofortified Gari in Nigeria: An Exploratory Study

Micronutrient deficiencies are a major public health challenge in sub-Saharan Africa, disproportionately affecting children under five and women of reproductive age. Deficiencies in vitamin A and iron are particularly prevalent, contributing to impaired cognitive development, weakened immune function, increased morbidi...

CRISPR - CAS MEDIATED GENOME EDITING FOR HUMAN DISEASE THERAPY: EMERGING APPLICATIONS, ADVANCEMENTS AND FUTURE PERSPECTIVES

CRISPR - Cas - mediated genome editing has emerged as a transformative technology with considerable potential for the treatment of human diseases. The ability of CRISPR-Cas systems to recognize specific nucleic acid recognize specific nucleic acid sequences and modify targeted genetic regions has expanded the possibili...

Base Editing as a Therapeutic Strategy for Autosomal Dominant Tubulointerstitial Kidney Disease caused by Pathogenic UMOD Variants

Autosomal dominant tubulointerstitial kidney disease caused by pathogenic UMOD variants (ADTKD-UMOD) is an untreatable monogenic kidney disease in which misfolded uromodulin is retained in the endoplasmic reticulum and exerts a toxic gain-of-function effect. Here we systematically evaluate the full spectrum of base edi...

The Genetic Discovery (GeDi) resource: Deep characterization of a multi-ancestry panel of human reference iPSCs for functional genomic studies

Human induced pluripotent stem cells (iPSCs) are essential tools for disease modelling, drug discovery, and functional genomics, yet the field remains anchored to a small number of reference lines, limiting our understanding of how genetic background shapes cellular phenotypes across human populations. Here we describe...

VASP cooperates with zyxin to organize mechanically competent adhesions

Abstract Focal adhesions (FAs) are dynamic macromolecular assemblies that anchor the actin cytoskeleton to the extracellular matrix via integrin receptors, thereby regulating cell morphology and migration. Here, we show that the vasodilator-stimulated phosphoprotein (VASP) cooperates with the mechanosensitive scaffold...

Development of an Efficient In Vitro Propagation Protocol for the Elite Bidah Pomegranate (Punica granatum L.) Landrace in Saudi Arabia

The Bidah pomegranate (Punica granatum L.) is an elite landrace native to Wadi Bidah in the Al-Baha region of Saudi Arabia. This cultivar is distinguished by its unique taste, high yield, genetic component, and phytochemical profile. In addition, its resilience to harsh environmental conditions aligns closely with nati...

CRISPR/Cas9 induced disruption of oca2 and tyr genes associated with pigmentation in Danio rerio

Targeted disruption of genes has been successfully carried out in several model and non-model fish species. In ornamental fish industries, Danio rerio (D. rerio) represents the most significant fish species and is available in several color varieties. Recently, CRISPR/Cas9 has been used to disrupt pigmented genes in se...

Functional dissection of ultraconserved non-coding regions present near the Hoxd complex in vertebrates

Ultraconserved non-coding elements (UCEs) exhibit near-perfect sequence conservation across vertebrate genomes, implying severe evolutionary constraint and essential biological functions. Near the vertebrate Hoxd complex, a master regulator of axial patterning and limb morphogenesis, three Conserved Regions (CR1, CR2,...

Artificial Intelligence as a Catalyst for Cell-Based Therapies in Retinal Disease

Retinal degenerative diseases, including age-related macular degeneration (AMD), retinitis pigmentosa (RP), and Stargardt disease, represent leading causes of irreversible vision loss worldwide, collectively affecting hundreds of millions of people. Cell-based therapies, particularly those leveraging induced pluripoten...