Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Beyond gene editing: the age of chromosome-scale genome engineering.

How advances in large DNA assembly, synthetic biology, and genome-wide validation are making chromosome-scale genome engineering an increasingly tractable experimental framework for understanding, building, and repairing genomes is discussed, while outlining the analytical requirements and current technical barriers th...

A multiplex genome editing pipeline for rapid combinatorial trait engineering.

Current de-extinction efforts center on editing the genomes of extant species to express key traits that evolved in closely related extinct species. These complex projects require advances in genetic engineering pipelines coupled with animal model production as an approach to test hypotheses about genotype and phenotyp...

High‐Content CRISPR Screening: Methods and Applications

Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 screening has become a central technology in functional genomics, enabling genome‐scale interrogation via pooled perturbations. Early CRISPR screens employed survival or simple phenotypic readouts to identify essential genes and drug resistance mec...

Precision genetic manipulation: CRISPR toolkits reshape plant biology.

Key CRISPR-derived tools are introduced, including base editors, prime editors, and programmable transcriptional and epigenetic regulators, and remaining challenges to stimulate future exploration of the full potential of CRISPR toolkits are outlined.

Programmable enzymes for targeted gene insertion.

EMERGING APPLICATIONS OF CRISPR–CAS9 BEYOND GENE EDITING

This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.