Genome Assembly and Genomic Characterization of Sanghuangporus mongolicus
TL;DR
These genomic data provide a resource for functional gene characterization and further biological studies of S. mongolicus and should not be interpreted as evidence of evolutionary gene-family expansion.
Abstract
Sanghuangporus mongolicus T. Bau is a wood-inhabiting medicinal fungus parasitic on Hemiptelea davidii, yet genomic resources for this species remain limited. Here, we generated and characterized a genome assembly from a verified monokaryotic isolate using PacBio long-read and Illumina short-read sequencing. The 34.79 Mb assembly comprised 13 contigs, with a contig N50 of 3.09 Mb and a GC content of 48.18%. BUSCO analysis recovered 98.3% complete orthologs, indicating high genome completeness. A total of 8471 protein-coding genes were predicted, including 491 genes annotated as carbohydrate-active enzymes (CAZymes), 447 transporter-related genes, and 123 cytochrome P450 genes. In addition, 16 secondary metabolite biosynthetic gene clusters were identified. A descriptive comparison with four published Sanghuangporus genomes showed that S. mongolicus had 491 CAZyme-related annotations (14.11 per Mb; 5.80 per 100 predicted protein-coding genes). Corresponding values for the other four species were 313–346 annotations, with 9.78–10.38 per Mb and 2.99–4.18 per 100 predicted genes. However, structural gene-prediction workflows differed among the five genomes, and no formal gene-family expansion analysis was conducted. Accordingly, these differences are reported descriptively and should not be interpreted as evidence of evolutionary gene-family expansion. These genomic data provide a resource for functional gene characterization and further biological studies of S. mongolicus.