The Evolution of Crucial Vertebrate Muscle Proteins

Abstract

Supplemental data failes to accompany the dissertation titled "The Evolution of Crucial Verterbate Muscle Proteins." Descriptions of files are provided below. Data S1: Myosin expression values and sample metadata from publicly available datasets. The file does not contain Lonchura striata expression data. NCBI accession numbers for both myosin loci and sample expression sets are provided. XLSX format. Data S2: Phylogenetic tree of cardiac myosin sequences with bootstrap support values. Tree is reflected graphically in Supplemental Figure S1. Newick format. Data S3. Multiple sequence alignment of vertebrate cardiac myosins. Alignment was used to generate the tree depicted in Supplemental Figure S1 and included in Data S2. FASTA format. Accession numbers included in sequence IDs. Data S4. List of vertebrate myosin sequences. The file also contains explanations of why sequences may have been excluded or descriptions of any manual edits made. CSV format. Data S5. Phylogenetic tree of avian cardiac myosins with bootstrap support values. This tree is depicted graphically in Figure 4C. Newick format. Data S6. Multiple sequence alignment of avian cardiac myosins. Alignment was used to generate the tree depicted in Figure 4C and included in Data S5. FASTA format. Accession numbers provided in sequence IDs. Data S7. Multiple sequence alignment of passerine and nonpasserine MYH6 and MYH6B sequences. Alignment was used to generate percent identity scores between passerine MYH6, nonpasserine MYH6, and passerine MYH6B. FASTA format. Data S8. Multiple sequence alignment used to generate 100% consensus sites between MYH6 and MYH6B sequences for passerine and nonpasserine birds. FASTA format. Data S9. Multiple sequence alignment of chicken myosin sequences from previous publications and NCBI reference genome. FASTA format. Data S10. Multiple sequence alignment of Sauropsid MYH15. Alignment was used to test for evidence of selection. FASTA format. Data S11. Phylogenetic tree of MYH15 sequences. Tree was used to test for evidence of selection. Newick format. Data S12. Expression data for Lonchura striata hearts. Multi-tabbed spreadsheet with proportion of MYH expressed, summarized MYH expression per sample, and NCBI genes used to quantify MYH expression. XLSX format.