TL;DR
This chapter describes how regions of interest can be created and used to assess distributions in tubular cells using two ImageJ macros, enabling efficient and precise identification of the pollen tube plasma membrane and tip, based on a few imprecisely marked points.
This chapter describes methods required for the generation of transgenic tobacco lines and for the expression level-independent quantitative characterization of PM domains and cytoplasmic regions at the pollen tube tip.
Abstract Cell division patterns shape developing organs, but their systematic analysis remains limited by the technical difficulty of existing detection methods. Here, we present a practical method for inferring recent daughter-cell pairs from static cell shapes. Although cell divisions have long been inferred by exper...
The final morphology of a plant is determined by much earlier events that occur during development. Meristems are critical for the development of the aerial parts of plants, such as leaves and stems. The morphology of these meristems, which can vary significantly across genetic backgrounds, correlates with phenotypic t...
By formalizing techniques often transmitted informally within laboratories, this protocol lowers the barrier to entry for maize meristem research, supporting downstream applications including imaging, gene expression analysis, and developmental phenotyping.
Live imaging of plant subcellular structures is key to deciphering the spatiotemporal bases of cellular processes, and their functional impact on growth and morphogenesis at various biological scales. Live imaging of plant cells essentially relies on expression of fluorescent markers labeling cells or subcellular struc...
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