TL;DR
A general protocol for the molecular grafting of two peptide affinity motifs onto OmpG, followed by purification and characterization of the engineered construct, to generate a single-channel multiplex sensor is presented.
This study provides a proof of concept for leveraging engineered living cells as multifunctional nanostructured templates and the modular, plug-and-play architecture of the biotin-streptavidin coupling interface highlights its potential for adaptation to clinical diagnostics and environmental monitoring.
In modern bioanalytical sensing, the integration of biorecognition elements with transducer platforms represents an established strategy for achieving selective detection. Among the available bioreceptors, proteins have been widely used for their ability to mediate highly specific interactions. However, proteins often...
Detecting target molecules in complex samples with both high sensitivity and selectivity remains a central challenge in biosensing, owing to low analyte abundance and interference from coexisting substances. Here, we present a membrane scaffold based on a ZZ-tag-displaying hepatitis B virus-derived L protein (ZZ-L me...
Immunoassays are critical for clinical diagnostics, yet their performance is restricted by the physical constraints of flat surfaces used to immobilize capture molecules which limit loading capacity and induce protein denaturation. Hydrogels provide immense volumetric molecular loading capacity but translating them acr...
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