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#gene editing

CRISPR/Cas9 Editing of Human Keratinocytes for Organotypic Modeling of Genetic Epidermal Disorders.

Anthony Coon Jessica L. Ayers Christopher Cole Afua Tiwaa A. Parihar Thomas Vincent J. Gudjonsson M. Sarkar C. L. Simpson
Aug 2026 · Methods in molecular biology · 0 citations
Medicine

TL;DR

Step-by-step methods for deploying CRISPR/Cas9 to engineer a TERT-immortalized human epidermal keratinocyte line (N/TERT-2G) to generate monoclonal heterozygous and homozygous knockout cell lines are described.

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Similar papers

Open access Aug 2026

CRISPR/Cas9-mediated generation of a homozygous MT4 knockout mouse embryonic stem cell line.

The CRISPR/Cas9 system is utilized to generate a homozygous Mt4 knockout (Mt4-/-) mouse embryonic stem cell (mESC) line that maintains normal morphology, pluripotency, and the ability to differentiate into all three germ layers.

Huan-Xin Zhou, Yine Li, Meiyan Jia et al. · 0 citations
Mar 2025

Genome-Wide CRISPR Screening Identifies Cellular Factors Controlling Nonviral Genome Editing Efficiency

A novel genome-wide CRISPR screening strategy that will facilitate the systematic engineering of novel nonviral genome editing delivery methods, where the identified novel gene hits can be further used to increase editing efficiency for other therapeutically relevant cell types.

Shivani Saxena, Meha Kabra, Amr A. Abdeen et al. · 2 citations
Open access Aug 2026

Genome-wide CRISPR screening identifies cellular factors controlling nonviral genome editing efficiency

To systematically map cellular factors constraining nonviral genome editing, influencing uptake and intracellular trafficking, we develop a genome-wide CRISPR screening platform linking perturbation of 19,114 genes to editing outcomes in human cells. We identify six negative regulators of delivery whose depletion incre...

Shivani Saxena, Meha Kabra, Amr A. Abdeen et al. · 0 citations
Open access Aug 2026

CRISPR/Cas9-based repair of a heterozygous HNF1A mutation in patient-derived hiPSCs

Human induced pluripotent stem cells (hiPSCs) represent a powerful platform for disease modeling, especially in monogenic diseases as they preserve the donor’s genetic background while enabling directed differentiation into disease-relevant cell types. This makes them highly suitable for studying disease mechanisms in...

D. Skoczek, J. Hohendorff, Maciej T. Małecki et al. · 0 citations
Review Aug 2026

CRISPR/Cas9-edited organoids as a platform for tailored research of colorectal cancer.

The potential of gene-edited organoids to discover the genetic and molecular mechanisms underlying colorectal cancer development, progression, and treatment resistance is explored.

Nikoleta Mojzesova, Petra Hladíková, Zuzana Kozovská et al. · 0 citations
#gene editing Open access Aug 2026

A PepFect14 analog improves non‐viral CRISPR delivery in primary human cells to facilitate genome editing and repair

A PepFect14 analog is employed to deliver high-fidelity Cas9-ribonucleoproteins and non-viral repair templates into primary human skin cells to mediate gene editing and repair targeting genes underlying the group of genetic skin blistering disorders epidermolysis bullosa (EB).

Alex du Rand, Courtney Masterson, Daniel J. Verdon et al. · 0 citations

Related blog posts

Microsoft Research Blog Oct 15, 2026

Proof-based general-purpose computing with Magnetar

Zero-knowledge proofs can verify computing results without rerunning workloads or exposing sensitive data. Magnetar helps bring them into the mainstream with support for standard programming languages, major speed gains, and quantum-resistant security. The post Proof-based general-purpose computing with Magnetar appeared first on Microsoft Research.

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