The hybrid yeast Saccharomyces pastorianus TUM 34/70 repurposes the pseudohyphal growth signaling network to establish a flocculation phenotype

The Role of FLO11 in Saccharomyces cerevisiae Colonization of The Role of FLO11 in Saccharomyces cerevisiae Colonization of the Lepidopteran Gastrointestinal Tract the Lepidopteran Gastrointestinal Tract

Comparative Genomic and Transcriptomic Analyses of 60Co-Mutagenized Scheffersomyces stipitis Strains: Identification of Candidate Genes Associated with High-Ethanol-Producing Xylose-to-Ethanol Fermentation

60 candidate key genes associated with high xylose-to-ethanol yield in S. stipitis are identified, predominantly involved in the cell cycle pathway, including CDC15 and PHO81.

Heterologous Expression of a Neurospora crassa Catalase Reprograms the Cellulase System and Enhances β-Glucosidase Production in Trichoderma reesei

Efficient saccharification of lignocellulose, the most abundant renewable carbon reservoir resource, is of great industrial importance. Trichoderma reesei is a premier cellulase producer, but its fermentation efficiency is often constrained by dual challenges: dissolved oxygen limitation and intrinsic oxidative stress. To address this, we engineered T. reesei to heterologously express a robust catalase gene (cat-3) from Neurospora crassa. The recombinant strain Tr-cNcat3 exhibited a 7.4-fold increase in extracellular catalase activity. Tr-cNcat3 showed an increase in total extracellular protein, resulting in markedly enhanced filter paper activity (FPA) and β-glucosidase activity compared to the control. Strikingly, this intervention specifically triggered a significantly higher expression of β-glucosidase, a known bottleneck in T. reesei’s cellulase system, particularly on bagasse and straw as the carbon source. Moreover, the ability of the supernatant to degrade cellulose substrates was improved. Our results reveal that overexpression of cat-3 in T. reesei could modify the cellulase cocktail by triggering a higher level of β-glucosidase. This study provides a novel and effective genetic engineering strategy to unlock the full industrial potential of T. reesei for cost-effective lignocellulosic biorefining.

High-cell-density fermentation of Komagataella phaffii enables the overproduction of a novel, broad-substrate endoglucanase, WcEG394 from Fuling (Wolfiporia cocos), for lignocellulose biorefining.

Recombinant WcEG394 efficiently hydrolyzed carboxymethyl cellulose, microcrystalline cellulose, and filter paper, and demonstrated broad substrate specificity toward xylans, pectin, chitin, agarose and agricultural residues such as corn cob and sugarcane bagasse.

Multistrategy metabolic engineering of Talaromyces pinophilus for α-amylase production from lignocellulosic biomass

Metabolic engineering of Talaromyces pinophilus through promoter optimization, multicopy integration, and protease deletion enables efficient α-amylase production from lignocellulosic biomass, achieving 26 712 U/mL in bioreactor fermentation.

The spatiotemporal-specific regulation of cell wall-related proteins promotes the secretion of pigments in Antarctic fungi Geomyces sp. wnf-18c.

The exogenous addition of xylose as a signal to initiate genetic editing achieved the spatiotemporal regulation of the target gene-activating gene editing that restricts growth only after the completion of the strain's growth phase, and raised the total pigment yield.