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High-throughput platforms for genetic perturbation screening using CRISPR/Cas9 in human iPSC-derived macrophages for drug discovery

Matteo Martufi Eleni Karagianni Tasos Papanikos Tim G. Ashlin Aikaterini Geladaki Benedetta Carbone Katja Remlinger Andres Quintero Moreno William G. Pembroke Charlie Haslam Nico Zinn Denise Vlachou Margarida Almeida Dimitrios Tsaousis S. Gade Bin Sun M. Maqbool Holger Franken Frederik Ziebell A. di Tullio Lewis J. Entwistle N. Srinivasan M. Lobera L. Mohamet
Sep 2026 · bioRxiv · 0 citations · 76 references
Biology

TL;DR

A scalable, semi-automated, and physiologically relevant hiPSC-derived macrophage model, rigorously characterised through deep comparative multi-omics is established and how genetic perturbations alter pro- and anti-inflammatory transcriptional signatures and significantly impact functional phenotypes are showcased.

DOI Semantic Scholar
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