Sustainable bioconversion of sugarcane bagasse into valorizable riboflavin by Microbacterium proteolyticum BWBTDIPO1, characterization and in-silico mechanistic pathway prediction
TL;DR
Valorizable Riboflavin obtained from waste SCB can be a green alternative to chemical Riboflavin synthesis as well as bulk SCB waste management, promoting the concept of microbe-mediated waste-to-wealth conversion, thus contributing to circular bioeconomy.
Abstract
Strategic bioconversion of lignocellulosic agro-wastes like sugarcane bagasse (SCB) into high-value Riboflavin is a rarely reported phenomenon. The present study explored a Riboflavin-producing bacterial strain, Microbacterium proteolyticum BWBTDIPO1 (GenBank Acc no.: PQ517523), isolated from the dumping area of Kolkata, West Bengal for its caliber to utilize SCB as a source of carbon and energy for the sustainable bio-production of the valuable nutraceutical under submerged fermentation (SmF) conditions. A strong association of bacterial growth (specific growth rate of μ = 0.325 h -1 ) with production of Riboflavin (yield of 397 ± 15.8 mgL - 1, which amounts to 19.85 ± 2.0 mg per gram of SCB after 78 h) was recorded. Yeast extract peptone mineral salt media (YPMSM) was found to be the most suitable medium for the production. UV-Vis spectrophotometry and thin-layer chromatography (TLC) with an R f value of 0.83 confirmed that the metabolite was Riboflavin. The biochemical assays revealed that the strain utilized SCB components, including lignin, cellulose, and hemicellulose. Further confirmation of biomass deconstruction was assessed via FTIR, XRD, and FESEM analysis, where reports ensured successful deconstruction of SCB by the bacterial isolate. To complement the experimental findings, KEGG-based in silico pathway analysis was performed to explore potential metabolic routes associated with lignocellulosic biomass utilization and carbon metabolism. The predicted pathways provide a hypothetical framework for understanding biomass deconstruction and require experimental validation. This is the first report of Riboflavin production from waste SCB via bacterial treatment (M. proteolyticum) , indicating the novel nature of the strain and the methodology employed. Valorizable Riboflavin obtained from waste SCB can be a green alternative to chemical Riboflavin synthesis as well as bulk SCB waste management, promoting the concept of microbe-mediated waste-to-wealth conversion, thus contributing to circular bioeconomy.