Similar papers
Jul 2026
A Novel Cold-Adapted and Heat-Resistant Alginate Lyase: Unveiling Its Characteristics and Mechanism of Thermostability.
A novel alginate lyase is identified, PpAly7A (26.75% sequence identity), which adapts to various pH, temperatures, salt concentrations, metal ions, and surfactants, and provides a viable molecular template for the rational design of thermostable industrial enzymes.
Luyao Tang, Shuhong Lin, Congyu Li et al.
· Journal of Agricultural and... · 0 citations
Aug 2026
Computer-aided multi-shell electrostatic remodeling of BhS7Xyl for enhanced activity and Thermostability.
Xylanases with high catalytic efficiency and environmental robustness are important for lignocellulosic biomass valorization, but many enzymes are rapidly inactivated under the alkaline and high-temperature conditions used in industrial processes. In this study, a computationally guided rational-design strategy was dev...
Chun-Lin Tan, Xin Yu, Lan-Xi Sun et al.
· International Journal of Bio... · 0 citations
Aug 2026
Insights into thermostability enhancement of a PL-5 alginate lyase through rational design.
Efficient utilization of natural alginate is restricted by its high viscosity. Alginate lyase degrades alginate via β-elimination to reduce viscosity, supporting its development and application. However, natural alginate lyases exhibit poor thermostability, limiting their application in high-temperature environments. E...
Shuhong Lin, Xin Li, Yiming Tang et al.
· Enzyme and Microbial Technol... · 0 citations
Jul 2026
Enhancing the Robustness of Nitrilase from Betaproteobacteria bacterium by Multistrategy Synergistic Evolution.
Molecular dynamics revealed that enhanced thermostability resulted from reduced loop flexibility, strengthened hydrophobic interactions, and new hydrogen bonds, which provided mechanistic insights into nitrilase thermostability.
Qi Zhu, Lanxin Xiao, Yijie Sun et al.
· Journal of Agricultural and... · 0 citations
Aug 2026
Optimizing acid stability and catalytic activity of Aspergillus flavus uricase (AfUOX) via surface charge engineering and B-factor guided design.
Uricase with improved acid stability is desirable for biomedical and biotechnological applications, yet enhancing the intrinsic acid tolerance of the enzyme while maintaining high catalytic activity remains a challenge. Here, we employed an integrated rational design strategy combining surface charge optimization and B...
Yu-Yue Li, Dan-Yao Zhou, Qing-Jiang Wen et al.
· International Journal of Bio... · 0 citations
Aug 2026
Multidimensional Computational Engineering of Pectate Lyase for Enhanced Thermostability and Moderately Improved Catalytic Efficiency.
This study provides a practical strategy for engineering thermostable pectate lyases with improved catalytic performance by developing a multidimensional consensus computational framework integrating sequence conservation, structural dynamics, and thermodynamic prediction to identify functional mutation hotspots in PcP...
Ziqi Hou, Gen Lu, Tong Shu et al.
· Journal of Agricultural and... · 0 citations