Open access
A strategy for decreasing prolamin content in rice endosperm through gene editing.
Zi-Ru Shen
Y. Li
Yuyan Huang
Tao Wang
Shijie Jian
Zihao Guan
Yuntong Feng
Xiangyuan Dang
Xinlan Shan
Shengyuan Sun
M. Guo
Zhibin Hu
Yihao Yang
Changjie Yan
Medicine
Abstract
Multiplex CRISPR editing of prolamin genes and phenotype-based screening revealed that reducing prolamin content in rice can increase lysine content and digestibility, improve taste. PROLM25 and PROLM26 are key genes for reducing prolamin content, and knocking out these two genes can achieve the same effect.