TL;DR
The findings suggest that the Ldhc and Dazl promoters used in this study are robust and are expressed in testicular germ cells, which can be used to study gene regulation during spermatogenesis to understand testicular physiology and male fertility.
A T/Brachyury promoter to CRISPR interference targeting CTNNB1, which encodes the canonical Wnt signaling mediator β-catenin, provides a proof of concept for combining a developmental promoter with a programmable intracellular regulator and identifies a strategy for separating circuit integration from unintended target...
Simple Summary Somatic cell reprogramming is an important technology for animal biotechnology, embryo engineering, and genetic improvement. However, the efficiency of reprogramming is limited by epigenetic barriers that restrict the activation of pluripotency-related genes. The long non-coding RNA XIST plays a central...
This Perspective discusses mechanisms enabling long-range enhancer–promoter communication and the potential advantages that distal enhancer positioning might confer on the establishment of developmental gene expression programs.
Background: Chemically inducible gene expression systems provide precise control over the temporal and spatial expression of genes. They are powerful tools for analyzing gene function during plant development and can effectively avoid the issues associated with transgene expression driven by constitutive promoters. The...
An mRNA’s cytoplasmic fate can be shaped by its transcriptional history, its modifications and associated protein complexes. Allele-specific expression (ASE), in which a gene’s two alleles are transcribed unequally, provides a natural experiment given that the alleles can differ in sequence or chromatin state, yet thei...
Housekeeping genes are widely used as experimental references for gene expression, yet many exhibit substantial variation within and between cell types. Using Zebrafish Meta Atlas Project (ZMAP), we found eef1a1l1 to be ubiquitously and highly expressed across zebrafish embryonic cell types, developmental stages, and s...
General Science
Zero-knowledge proofs can verify computing results without rerunning workloads or exposing sensitive data. Magnetar helps bring them into the mainstream with support for standard programming languages, major speed gains, and quantum-resistant security. The post Proof-based general-purpose computing with Magnetar appeared first on Microsoft Research.
General Science
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