Immunoassays are essential tools in clinical diagnostics, food safety surveillance, and environmental monitoring; however, a persistent discrepancy exists between the theoretical performance of bioreceptors and their practical efficacy in sensor devices. This performance gap is largely rooted in the stochastic nature o...
The reliable electrochemical detection of immunoglobulin G (IgG) in complex biological fluids is severely hampered by nonspecific protein fouling and enzymatic degradation. Inspired by natural lasso peptides, we engineer a multifunctional lasso-like peptide (LaP) composed entirely of D-amino acids that uniquely integra...
We developed a site-directed immobilization strategy exploiting the competitive inhibitor Remazol Brilliant Blue R (RB) to orient horseradish peroxidase (HRP) on functionalized glass surfaces. RB was covalently bound to poly(ethylene-alt-maleic anhydride) (PEMA)-coated substrates, enabling HRP binding via its active si...
Anne Muschter, S. Rosencrantz, T. Chouki et al.· Molecules· 0 citations
A robust computational strategy for engineering high-affinity, stable bispecific aptamers for enhanced food safety monitoring by introducing a novel C-type configuration utilizing poly-T linkers with complementary terminal poly-A sequences, facilitating self-assembly via A-T base-pairing.
Shuang Jiang, Yue-Xiang Ren, Xue Wang et al.· Analytica Chimica Acta· 0 citations
Detecting target molecules in complex samples with both high sensitivity and selectivity remains a central challenge in biosensing, owing to low analyte abundance and interference from coexisting substances. Here, we present a membrane scaffold based on a ZZ-tag-displaying hepatitis B virus-derived L protein (ZZ-L me...
This method reduces the recovery loss caused by decreased affinity from payload conjugation and delivers comparable signals for trastuzumab, T-DM1, and T-DXd, which provides a novel approach to address accuracy issues arising from affinity changes due to ADC dynamics in vivo.
