AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
This chapter describes two in vitro assays designed to assess the hematopoietic stem/progenitor cell (HSPC)-supporting capacity of primed MSCs, and the other is specifically aimed at their ability to support the growth of primitive HSCs.
A protocol to isolate and expand MSCs from human umbilical cord tissue and characterize their cell surface protein expression by flow cytometry, self-renewal potential by colony-forming assay, and tri-lineage differentiation capacity is outlined.
Background/Objectives: Mesenchymal stromal cells (MSCs) isolated from neonatal sources have been proposed in clinical cell therapy to enhance hematopoietic stem cell transplantation due to their hematopoietic support capacity. Here we evaluated this capacity, particularly the lymphopoietic potential that remains poorly...
These cells can be isolated from a wide range of human tissues, including bone marrow (BM-MSCs), adipose tissue (AD-MSCs), dental tissues, such as dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs), as well as umbilical cord cells (UC-MSCs), including Wharton’s jelly–derived MSCs (UC-MSCs-WJ) a...
Background: Mesenchymal stromal cells (MSCs) are among the most extensively studied candidates for cell-based therapy. The homing capacity of MSCs is a critical factor influencing the efficacy of MSC-based therapies. This study aimed to investigate the homing of placenta-derived mesenchymal stromal cells (PD-MSCs) in v...
This integrated bone marrow organoid platform provides a physiologically relevant system for studying human hematopoietic development and immune cell differentiation and offers a scalable foundation for regenerative medicine and immunotherapy applications.
BACKGROUND Acute graft-versus-host disease (aGVHD) remains a leading cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The development of methods that preserve graft-versus-leukemia (GVL) activity while preventing aGVHD remains a long-sought goal. Recent studies sugg...
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