Specimen-Dependent Sampling and Signal Limitations Govern the Effectiveness of Low-Magnification Super-Resolution in Cryo-EM

Review Aug 2026

ONE microscopy.

One-step nanoscale ExM is developed, which combines ExM with fluctuation-based super-resolution analysis to enable the direct visualization of individual protein shapes, using conventional fluorescence microscopes, a capability that was previously limited to cryo-electron microscopy and averaging-based techniques.

Ali H. Shaib, Mohamad Mahdi Alawieh, S. Rizzoli · Nature Protocols · 0 citations

Open access Aug 2026

Imaging large fields-of-view at high resolution in cryo-ET with square beam montaging

Visualizing macromolecules within their native cellular context by cryo-electron tomography (cryo-ET) is fundamentally limited by the trade-off between field of view and resolution: Capturing high-resolution information about biomolecules requires high magnification, which restricts the field of view and obscures the c...

Eugene Y. D. Chua, Hamidreza Rahmani, James Zhen et al. · bioRxiv · 1 citation

Conference Sep 2026

DIP-based super-resolution quantitative phase imaging via synthetic aperture enhancement

Quantitative phase imaging (QPI), as a label-free microscopic imaging technique, provides quantitative optical information of cells and their subcellular structures, holding significant value in biomedical research. However, existing methods still face a notable trade-off between resolution and data efficiency. Differe...

Gui-Feng Lu, Zhuo-Shi Li, Qing-Yang Fu et al. · Global Intelligent Industry... · 0 citations

Open access Aug 2026

SPIFFI enables single-shot super-resolution and multidimensional imaging

Fluorescence super-resolution microscopy has advanced optical imaging into the nanoscale regime, transforming biological and interdisciplinary research. However, wide-field super-resolution techniques often compromise temporal resolution, thereby limiting the capture of rapid and transient biological events in living s...

Wei Guo, L. Feletti, A. Radenović · Nature Methods · 0 citations

Sep 2026

Phase-resolved near-field optical microscopy approaching the shot-noise limit

Scattering-scanning near-field optical microscopy (s-SNOM) is a powerful probe of molecular and quantum systems, using optical field enhancement at the apex of a scanning probe to provide few-nanometer resolution and sub-attomolar sensitivity. However, small sample volumes and associated small signals remain challengin...

Patti Rizzo, Div Chamria, Johna Joseph et al. · Applied Physics Letters · 0 citations

Open access Sep 2026

Widefield-excitation two-photon single-pixel microscopy without temporal-focusing

Two-photon microscopy (2PM) constitutes a key-enabling technology for imaging in weakly scattering specimens, delivering high-contrast signals through the quadratic dependence of fluorescence on excitation intensity. Extending 2PM to widefield or scan-less formats is attractive for rapid functional imaging. To maintain...

H. Tobón-Maya, S. Zapata-Valencia, Gladys Mínguez-Vega et al. · Journal of Physics: Photonic... · 0 citations