TL;DR
One-step nanoscale ExM is developed, which combines ExM with fluctuation-based super-resolution analysis to enable the direct visualization of individual protein shapes, using conventional fluorescence microscopes, a capability that was previously limited to cryo-electron microscopy and averaging-based techniques.
Lattice light-sheet microscopy (LLSM) enables gentle, rapid, volumetric fluorescence imaging of living biological systems. The technique employs Bessel beam interference to produce a light-sheet which retains its thickness over a relatively large field-of-view compared to other light-sheets. In the decade since its com...
Abstract Over the past decade, biological imaging has been revolutionized by the convergence of two distinct technologies: expansion microscopy (ExM) and light sheet fluorescence microscopy (LSFM). ExM bypasses the diffraction limit of light by embedding biological specimens in a swellable polyelectrolyte hydrogel and...
Weak, reversible interactions underpin biomolecular recognition, and single-molecule fluorescence (smF) imaging techniques can provide unprecedented insight into those biological processes. Unfortunately, such studies often require micromolar concentrations of fluorophore-labeled biomolecules, which is beyond the acces...
Super-resolution fluorescence microscopy (SRM) has emerged as a powerful tool for polymer characterization, providing nanometer-scale spatial resolution and molecular specificity. Recent advances in probe design, labeling strategies, and imaging methodologies have enabled SRM to move beyond structural visualization to...
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