TL;DR
This chapter describes optimized RNA-seq workflows for studying PNA-CPPs in Escherichia coli and Salmonella enterica; discusses considerations for RNA isolation, sequencing strategies, and data analysis; and emphasizes the importance of robust, reproducible pipelines for reliable data interpretation.
Single-cell RNA sequencing (scRNA-seq) is fundamentally reshaping the research paradigm of mosquito biology and host–pathogen interactions via its high-resolution transcriptomic profiling capability. This review first outlines the technical workflow and data analysis strategies of scRNA-seq, then highlights key applica...
The rapid emergence of antibiotic-resistant bacteria has created an urgent need for innovative therapeutic strategies that go beyond conventional small-molecule antibiotics. Among the most promising approaches is the use of nucleic acid-based therapeutics, particularly antisense oligonucleotides (ASOs), which offer hig...
The ribosome–mRNA interface is identified as an intracellular determinant of antibacterial PNA susceptibility and rpsLI82N alters uS12-dependent 30S assembly and initiation-complex dynamics, thereby changing the effective occupancy of mRNA translation-initiation regions.
Gram-negative bacteria are a major cause of antibiotic-resistant infections due to their highly impermeable cell envelope which contains lipopolysaccharide (LPS) and prevents antibiotics from entering the cell. LPS biogenesis relies on the activity of the essential LPS transport machinery (Lpt), which represents an att...
Bacterial persistence enables a phenotypically tolerant subpopulation to survive lethal antibiotic exposure, contributing to the recalcitrance of chronic infections and facilitating the evolution of heritable resistance. Despite its importance, a systems-level, quantitative understanding of how transcriptional regula...
Antibiotic resistance is an increasing threat to modern medicine that calls for the discovery of new antibiotics. RNA targeting is a promising approach for antibiotic development. In particular, antisense antibiotics, dubbed ASObiotics, are an exciting class of oligomers that show promise as powerful antibiotic agents....
We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.
