This work uncovered an unprecedented role of V-ATPase in shaping the macropinocytic cup in the pathogenic amoeba Entamoeba histolytica and highlights the novel role of V-ATPase in directly driving actin polymerization, in conjunction with phosphatidic acid, to shape the macropinocytic cup.
Key mechanistic steps coordinating ATP-driven MreB polymerization and turnover are established and provide a basis for a complete MreB assembly–disassembly cycle and for further elucidating how MreB dynamics contribute to cell-wall organization.
Alba de San Eustaquio-Campillo, C. Cornilleau, Sana Afensiss et al.· bioRxiv· 0 citations
Data support a canonical function for the Leishmania v-ATPase in lysosome acidification and autophagy, which is essential for parasite differentiation, and identify the poorly characterized Leishmania CVC as another major site of v-ATPase concentration.
Ç. Alagöz, Andreia Albuquerque-Wendt, E. Gluenz· bioRxiv· 0 citations
It is found that both nucleotide and substrate polypeptide binding restrict the conformational landscape sampled by katanin and ClpB, with ligand-specific conformations observed in the latter case.
This work shows that SWAP70 is rapidly recruited to regions of intense actin remodeling at the synaptic interface, where it forms a stable network with slower turnover than filamentous actin, indicating that SWAP70 cooperates with the linear actin filament to help stabilize the cortical cytoskeleton.
A three-dimensional ultrastructural analysis reveals previously unrecognized intermediate stages in the formation of primary cilia and identifies the endocytotic protein GRAF1 as being essential during the early stages of ciliogenesis and for the delivery of plasma membrane-derived material to the developing ciliary me...
Kerstin N. Schmidt, Korbinian Buerger, Olga Maier et al.· Nature Communications· 0 citations
This work uncovers the molecular basis by which formin-actin-myosin working system generates force driving massive secretory vesicles for directional transport during pollen germination.
