Directional transport of massive secretory vesicles to the germination site is a key process for pollen germination, but the molecular mechanism remains unclear. Here, we find that Myo11C proteins, Myo11C1 and Myo11C2, act redundantly. This functional redundancy is evidenced by impaired vesicle tethering efficiency and aberrant F-actin organization in myo11c1 myo11c2 double mutant. Myo11C1 unexpectedly colocalized with AtFH5-located secretory vesicles (AtFH5-SVs), rather than with actin filaments. Simulated modeling and truncation mutation analyses show that Myo11C binds actin filaments nucleated from AtFH5-SVs and exhibits plus-end–directed motility, leading to its polarized localization at F-actin plus-ends. Upon dissociating from F-actin, Myo11C directly interacts with AtFH5-SVs to promote their aggregation. E447 is identified as a critical residue for Myo11C1 binding to F-actin, while R1377 and R1452 are conserved residues essential for Myo11C1’s interaction with secretory vesicle–located SEC5B. Together, our work uncovers the molecular basis by which formin-actin-myosin working system generates force driving massive secretory vesicles for directional transport during pollen germination.
The recruitment and activation of abscission machinery following mitosis is tightly spatiotemporally regulated, yet the mechanisms underlying this remain poorly understood. We find that RNA localization and local translation at the midbody governs when and where abscission-regulating proteins are expressed. The 3′ UTR...
Katherine F. Vaeth, Andrew J. Neumann, Frances D H Zorensky et al.· bioRxiv· 0 citations
Summary Aspergillus nidulans hyphae grow by apical extension fueled by apex-directed exocytosis. Vesicles arriving at the tip are concentrated by MyoE (myosin V) in a membraneless organelle, the Spitzenkörper, before undergoing a second round of transport to the plasma membrane. Sec4, which is recruited to the vesicles...
Juan Fernández-Carrillo, Mario Pinar, Areti Pantazopoulou et al.· iScience· 0 citations
Auxin exerts diverse physiological effects through local maxima and tissue-scale gradients established by directional transport, which depends on the plasma membrane (PM) distribution and vesicular trafficking of auxin transporters. Cortical microtubules are positioned beneath the PM and have been implicated in traffic...
Jia-Qin Deng, Jing Qin, Pan-Pan Wang et al.· Science Advances· 0 citations
This work uncovered an unprecedented role of V-ATPase in shaping the macropinocytic cup in the pathogenic amoeba Entamoeba histolytica and highlights the novel role of V-ATPase in directly driving actin polymerization, in conjunction with phosphatidic acid, to shape the macropinocytic cup.
Eukaryotic cells contain multiple biochemically distinct actin filament networks, which enable versatile functions of actin in a range of cellular processes. Yet, the mechanisms by which specific actin filament networks are assembled in a common cytoplasm remain elusive. Here, we investigated how functionally distinct...
Le-Chua Xiang, Parijat Biswas, H. Wioland et al.· bioRxiv· 0 citations
Microtubule-based organelle transport is essential for organelle positioning within cells and defines the architecture and function of highly polarized cells, like animal neurons and fungal hyphae. Early endosome transport depends on kinesin-3 motors and on cytoplasmic dynein, which binds to this organelle via Hook ada...
Fernando Hernández-Sánchez, Yovanna A Pardo-Fermín, Sara Schroder et al.· Biochimica et biophysica act...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
