Methods for the Evasion of Restriction-Modification Systems During Genetic Engineering of Fusobacterium Species.

Martha A. Zepeda-Rivera Elsa F. McMahon Kaitlyn N. Lewis Rutika P. Gavate Falk Ponath C. Johnston

2026 · Methods in molecular biology · Vol 3055, pp. 81-92 · 0 citations

Medicine

TL;DR

This chapter describes strategies to evade RM defenses and improve transformation efficiencies across diverse Fusobacterium lineages, employing a sequence-based "RM-silencing" method that has enabled successful delivery of replicative plasmids, linear recombination templates, and transposon cassettes into previously intractable Fusobacterium clinical isolates.

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