A practical framework for isoform-level analysis in single-cell CRISPR screens is provided, identifying current capabilities and limitations, and providing a practical framework for isoform-level analysis in perturbation studies scale.
This review examines the experimental and computational foundations of scLR-seq, including platform selection, library design, cell barcode and unique molecular identifier recovery, transcript discovery, and isoform quantification, and summarize emerging insights into isoform usage, alternative splicing, transcription...
The first single cell long-read sequencing dataset of fetal human retina can help elucidate the role of alternative splicing in retinal development and guide the design of transcript-specific gene therapies for retinal regeneration.
The findings conservatively indicate that Smart-Seq may not be good at analyzing molecular diversity and that the results need to be interpreted with caution.
Y. Duan, Jiyao Liu, Shiwen Xu et al.· Nucleic Acids Research· 1 citation
This work compares the performance of multiple KRAB domain systems, develops an updated CRISPRi-specific on-target scoring scheme, and quantitatively characterize off-target effects associated with seed-sequence patterns.
Smriti Srikanth, Fengyi Zheng, Laura M Drepanos et al.· Cell Genomics· 0 citations
This work establishes SBX and the AXELIOS 1 as a transformative platform for high-scale single-cell isoform sequencing and demonstrates that this consensus approach successfully captures the vast isoform diversity of single-cell libraries and enables the accurate measure of differential isoform expression across distin...
Christophe H. Georgescu, Ghamdan Al-Eryani, Allison Brookhart et al.· bioRxiv· 0 citations
This work presents CRISPRLungo, a computational pipeline specifically designed for long-read amplicon sequencing of gene edited samples that incorporates unique molecular identifier-based error correction and statistical filtering to distinguish true editing events from background noise, enabling robust detection of sm...
Gue-Ho Hwang, Benjamin Vyshedskiy, Timothy M. Barry et al.· Nature Biomedical Engineerin...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
