Jul 2026· Journal of Agricultural and Food Chemistry· Vol 74 31, pp.
24542-24552
· 0 citations· 26 references
Medicine
Abstract
Caffeic acid (CA) is a valuable phenylpropanoid with applications in food, pharmaceutical, and chemical industries. Microbial production of CA is often limited by the terminal hydroxylation catalyzed by 4-hydroxyphenylacetate 3-monooxygenase (HpaBC). Here, we constructed a de novo CA biosynthetic pathway in Escherichia coli and enhanced production through systematic metabolic and spatial engineering. Optimization of l-tyrosine supply and HpaBC expression increased CA production to 61.0 mg/L. However, further enhancement of glucose uptake and precursor supply was insufficient, indicating that hydroxylation remained a major limitation. To address this, HpaBC was relocated to the periplasm via the Tat pathway, increasing CA production 4.9-fold to 299.1 mg/L with reduced byproduct formation. Further lpp+14 mediated periplasmic remodeling and fermentation optimization increased shake-flask production to 463.7 mg/L. Finally, fed-batch fermentation achieved 5.1 g/L CA in a 3 L bioreactor. This study highlights periplasmic engineering as an effective strategy for improving oxidation-dependent phenylpropanoid biosynthesis.
L-Tyrosine is an important aromatic amino acid widely used in dietary supplements and as a precursor for high-value food ingredients. Microbial fermentation presents a sustainable and green production route, but achieving high titers remains challenging due to incomplete understanding of metabolic regulation. In this s...
Ziyue Zhao, Anying Ji, Xue-Tuan Wei et al.· Journal of Biotechnology· 0 citations
Results indicate that catechol, not PCA, is the principal bottleneck in muconate production via the PCA decarboxylation route originally demonstrated by Draths et al., refining the understanding of pathway limitations and offering new strategies for improving rate, yield, and strain resilience in muconate bioproduction...
Alissa C. Bleem, Tracy L. Hodges, Torrey M Lind et al.· bioRxiv· 2 citations
Xylitol is a highly functional sweetener with extensive applications. Sustainable biosynthesis from glucose is desirable yet metabolically challenging. Here, we engineered Yarrowia lipolytica as a cell factory by constructing a core biosynthetic route via combinatorial screening and multicopy integration of d-arabitol...
Bingbing Liu, Xi Yao, Jianping Lin et al.· Journal of Agricultural and...· 0 citations
Trehalose is a functional disaccharide widely used in the food, pharmaceutical, and cosmetic industries. It is industrially produced via a dual-enzyme process involving maltoligosaccharide trehalose synthase (MTSase) and maltoligosaccharide trehalose hydrolase (MTHase), with Escherichia coli (E. coli) serving as the ex...
Jianghua Chen, Yu-Jue Wang, Qiang Wang et al.· Fermentation· 0 citations
Vanillin is an important flavor compound widely used in the food, fragrance, and pharmaceutical industries. Current biotransformation processes from ferulic acid or eugenol are limited by high substrate cost and low carbon efficiency, motivating de novo biosynthesis from glucose. This study employed Escherichia coli as...
Yue Wang, Tian-Jie Han, Yan-Xiang Bao et al.· Biotechnology and applied bi...· 0 citations
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