Transcriptomic and metabolomic analyses revealed the action mechanism of nesfatin-1 gene on glucolipid metabolism in early development stage of largemouth bass.

Abstract

Nesfatin-1 has biological roles including the suppression of food intake and the regulation of glucose and lipid metabolisms. However, th8e information available regarding nesfatin-1 in the glycolipid metabolism in the early development stage of fish is still limited. In order to investigate the role of the nesfatin-1 gene in the early development stage of the largemouth bass (Micropterus salmoides), the nesfatin-1 gene was inhibited using siRNA interference technology. Then, we evaluated their mRNA expression levels, transcriptomes and metabolomes. The mRNA expression levels of nesfatin-1 gene were appreciably decreased at 48 h,72 h and 96 h after injection of nesfatin-1 siRNA in the early development stage. The omics results revealed that the nesfatin-1 gene was interfered to induce 1833 differentially expressed genes (DEGs) and 2370 differentially expressed metabolites (DEMs). Bioinformatic analysis enriched the most affected molecular pathways (sphingolipid metabolism, fatty acid elongation, amino sugar and nucleotide sugar metabolism and biosynthesis of unsaturated fatty acids) and metabolic pathways (biosynthesis of unsaturated fatty acids, sphingolipid metabolism and amino sugar and nucleotide sugar metabolism) in early development of largemouth bass. In amino sugar and nucleotide sugar metabolism, increased expression levels of genes such as chic, chs1, and gck genes, alongside decreased expression levels of the chia.1 gene, resulted in significantly elevated concentrations of N-Acetyl-D-glucosamine, beta-d-Fructose 6-phosphate, beta-d-Fructose, D-Mannose 6-phosphate, d-Glucose, d-Glucose 1-phosphate, UDP-glucose, UDP-glucuronate, whilst the concentration of UDP-N-acetyl-alpha-D-glucosamine was markedly reduced. Therefore, the nesfatin-1 gene could influence the early development stage of largemouth bass by affecting signaling pathways associated with glycolipid metabolism. Our findings further expand the molecular mechanisms of nesfatin-1 gene, and provide further theoretical support for the initial breeding and feed adaptation of largemouth bass.