Programmable Allosteric DNAzyme Coupled with CRISPR/Cas12a System for Multiplexed and Sensitive Detection of Extracellular Vesicle Derived MicroRNAs

Xiang-Lan He Le Wang Cong Zhang Meng-Meng Pan Ying Ma Jia-Qi Du Linjiao Yang Ming Wang Ming Jiang Xu Yu Li Xu

Aug 2026 · bioRxiv · 0 citations · 18 references

Biology

TL;DR

A previously unrecognized feature of CRISPR/Cas12a is identified, in which incorporation of ribonucleotides into single stranded DNA targets modulates Cas12a activation efficiency, revealing a hybrid DNA/RNA-dependent regulation of Cas12a activity.

DOI Semantic Scholar

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