Tracking Surface Protein Assembly and Peptidoglycan Biosynthesis in Fusobacterium nucleatum.

Discovery of a regulatory node that coordinates cell envelope assembly in mycobacteria

Mycobacteria possess a complex double-membrane cell envelope critical for survival and pathogenesis. Proper assembly of this architecture requires the biosynthesis and transport of major components, including arabinogalactan (AG) polysaccharides and mycolic acids (MAs), but how these processes are effectively coordinat...

Peptidoglycan Remodeling in Gram-Negative Bacteria: From Stress Adaptation to Antibiotic Tolerance and Therapeutic Targeting

The peptidoglycan (PG) sacculus of Gram-negative bacteria is continuously reorganized by a diverse enzymatic repertoire, including lytic transglycosylases, endopeptidases, carboxypeptidases, amidases and LD-transpeptidases, that operate alongside PG synthases to maintain envelope integrity throughout the cell cycle. Th...

Caenorhabditis elegans as a Model Host for Fusobacterium nucleatum.

Caenorhabditis elegans is presented as a versatile and tractable model for investigating F. nucleatum virulence determinants and offer a valuable platform for advancing the understanding of its pathogenic potential.

The SigD regulon of Mycobacterium abscessus determines cell envelope composition and antibiotic susceptibility

Results demonstrate σD is active during logarithmic growth in rich media where it regulates the expression of envelope-associated genes that influence envelope permeability and basal level susceptibility to hydrophobic antibiotics; its activity further increases in response to cell envelope stress, presumably promoting...

Oxygen-responsive bacterial glycosphingolipid links symbiont fitness and immune development in neonatal host

Summary Symbiotic gut bacteria must re-establish themselves in every host generation, yet the molecular strategies enabling this inheritance remain poorly understood. Here we show that Bacteroides fragilis uses a membrane glycolipid, alpha-galactosylceramide (BfaGC), to colonize the neonatal gut. Genome-wide fitness pr...

Isolation and Proteomic Characterization of Outer Membrane Vesicles in Fusobacterium nucleatum.