Hung Ton-That

A Mouse Model for Fusobacterium nucleatum-Mediated Experimental Periodontitis.

Modeling Fusobacterium nucleatum-Driven Colorectal Cancer in Apc-Mutant Mice.

A two-factor authentication mechanism licenses pilins for pilus assembly in gram-positive bacteria.

A "two-factor authentication" mechanism for pilus assembly in gram-positive bacteria is defined: class C sortases first verify pilin identity by forming a membrane-embedded interface that activates the enzyme, then they recognize the LPXTG motif to initiate loading and crosslinking.

A 3D Tumor Spheroid Platform to Model Fusobacterium nucleatum-Induced Tumorigenesis.

A detailed protocol is presented in which colorectal cancer cells are seeded into ultralow attachment plates and co-cultured with F. nucleatum either prior to spheroid formation, to model early tumor development, or after spheroids have been established, to model tumor progression.

Tracking Surface Protein Assembly and Peptidoglycan Biosynthesis in Fusobacterium nucleatum.

A fluorescence-based labeling approach using fluorescent D-amino acids (FDAAs) to simultaneously track peptidoglycan biosynthesis and the localization of FadA in F. nucleatum is presented, providing a versatile platform for investigating the dynamic interplay between virulence factor localization and cell envelope remo...