Indication for FLT3 and CD34 Among Acute Myeloid Leukemia in Sudanese Patients
TL;DR
M Males were less than females, CD34 was detected among 21% and FLT3 mutation detected among 18% as wild, those considered as poor prognosis phase, and MPFC applicable to more than 90% of AML cases, offering broad utility compared with PCR-based molecular MRD testing.
Abstract
Background: Multiparametric flow cytometry (MPFC) is an effective method for AML diagnosis, classification, characterization, and MRD monitoring. Abnormal surface and cytoplasmic antigen patterns can form leukemia-associated immunophenotypes (LAIPs), enabling sensitive MRD detection. MPFC is applicable to more than 90% of AML cases, offering broad utility compared with PCR-based molecular MRD testing. AML outcomes vary widely, and prognosis is influenced by age, performance status, and cytogenetic abnormalities. CD34 is an important cell-surface glycoprotein used in AML immunophenotyping. CD34 contributes to cell adhesion and may facilitate interactions between hematopoietic stem/progenitor cells and the bone marrow stromal microenvironment. The FLT3 protein is primarily expressed on early hematopoietic progenitor cells, especially CD34-positive cells in the bone marrow, highlighting its important role in the initial stages of blood cell formation. Although FLT3 is also present in various other tissues, its major function is to act as an important regulatory receptor in hematopoiesis. This study aimed to detect CD34 and FLT3 among Sudanese patients with AML in order to assist in diagnosis and prognosis. Methodology: 100 AML patients were enrolled in this study, they were 40% males and 60 females. Complete blood count was assessed via Beckman Coulter device and CD34 was detected through flow cytometer assessment and FLT3 was detected through molecular detection through PCR. Data analysis was conducted by statistical package of social science. Patients were recruited from Khartoum center for Nuclear and Isotope therapy -Sudan. Result: Out of collected data of patients’ files, CD34 detection, which was conducted via flow cytometer, it showed 21% with no CD34 and 79% with presence of CD34. FT3 mutation also detected 18% with wild type and 82% as mutant. Conclusion: Males were less than females, CD34 was detected among 21% and FLT3 mutation detected among 18% as wild, those considered as poor prognosis phase.