A unifying biophysical model is presented that provides a physical explanation of how changes in the solution environment modulate interactions between polymer chains within the brush layer and predicts that tuning the ionic strength of the extracellular space, along with the properties of the membrane-tethered polymer...
Ke Xiao, Feng Yuan, Wade F. Zeno et al.· bioRxiv· 0 citations
The self-assembly of the cholesterol-dependent cytolysin Perfringolysin O (PFO) into stable transmembrane nanopores composed of 46 ± 9 monomers is reported, enabling, for the first time, resistive-pulse-based characterization of intact virus particles and ribosomes with a biological nanopore.
Andela Vracar, Veneta Salyahetdinova, S. Balog et al.· bioRxiv· 0 citations
It is proposed that, due to fundamental biophysical differences between the dendritic cytosol and the postsynaptic density, there are two distinct classes of IDPs functioning in these different environments and these differences should be considered when optimizing force fields and parameters of water models for studyi...
M. Shokhen, A. Albeck, N. Levy et al.· Biophysical Journal· 0 citations
The endoplasmic reticulum (ER) is a membrane-bound organelle found in all eukaryotic cells. This organelle has a fascinating morphology, which involves a network of membrane nanotubes and three-way junctions connecting three nanotubes. Previous reconstitution studies were based on proteo-liposomes with a size of 100 to...
Cade Vallero, Shreya Pramanik, Tsu-Wang Sun et al.· bioRxiv· 0 citations
This work presents the first fully automated HDX−MS platform incorporating a two-stage delipidation workflow and underscores the importance of studying membrane proteins in native lipid environments.
Charlotte Guffick, J. R. Rincon Pabon, Damon Griffiths et al.· Analytical Chemistry· 0 citations
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