Metabolic and redox transcription after brief psilocin exposure in human iPSC-derived cortical neurons

Abstract

View the results in the interactive transcriptomic atlas. This record is an independent secondary analysis and derived research object. It is not the original Schmidt experimental dataset or the original Hoffrichter/Schmidt analysis. Original experiment and data generation: Schmidt et al. generated the human iPSC-derived cortical-neuron data and reported the experiment in Psilocin fosters neuroplasticity in iPSC-derived human cortical neurons (2026), doi:10.7554/eLife.104006. The associated source research data are deposited at Dryad, doi:10.5061/dryad.xsj3tx9w3, under Dryad's CC0 framework. Original analysis: the Hoffrichter/Schmidt RNA-seq analysis code is pinned at Git commit c565709affafbea09406bd7b42fe494f08db6cce and preserved by Software Heritage as swh:1:dir:c847a8a51074c59d651e3258cf1355936e921048. This secondary analysis: Christopher B. Germann independently reanalysed the public RNA-seq data. This archive contains the newly generated reproducibility code, frozen specifications, derived tables, figures, tests, and a static copy of the interactive atlas. Schmidt et al. are cited as the source-study authors; they are not creators of this research object. Version 1.2.0: includes the separately specified post hoc limma-voom CAMERA sensitivity across all five original pathways and three contrasts, full outcomes under estimated and documented fixed correlation, diagnostics and direct runtime verification. Original primary results and freezes are unchanged. Upward OXPHOS direction is retained at both days, but competitive significance depends on CAMERA correlation settings and their reference distributions: estimated correlation gives q=0.587 at both days; fixed 0.01 gives q=5.84e-8. The Bayesian extension remains included; expression-shift magnitudes are uncertain, particularly at Day 3. The analysis identifies a broad OXPHOS-centered transcriptional signature at both sampled days. It reports the complete primary pathway family, gene-level estimates, leading edges, prespecified robustness checks, and bounded exploratory analyses. Unknown control harvest composition limits treatment attribution at either day and prevents treatment-specific temporal inference. Statistical significance is not independent of analytical assumptions. Transcriptional enrichment does not establish respiration, ATP production, redox flux, DNA-repair activity, or telomere preservation. Licensing: source Dryad research data are distributed under CC0. This archive does not relicense them. Newly created software is MIT licensed; newly created documentation, figures, and derived result tables are CC BY 4.0 unless a path-level notice states otherwise. Upstream components retain their original terms. This is a mixed-license archive; exact rights and notices are documented in LICENSE.md, REUSE.toml, FILE_LICENSES.json, and THIRD_PARTY_NOTICES.md. Secondary-analysis repository: GitHub.