cerevisiae-based platform for rapid production and evaluation of Eukaryotic Nutrient transporters and transceptors for biochemical studies and crystallography
A new effective S. cerevisiae expression system is developed that may be used for production of high-quality eukaryotic membrane proteins for functional and structural analysis.
A case-study evaluation of a pBR322-derived expression system, previously applied to therapeutic peptides and insulin analogs, in combination with fed-batch cultivation for recombinant production of methionine aminopeptidase in E. coli, evaluating the performance of this system for methionine aminopeptidase as a stress...
G. Kuznetsov, Marina Yarovikova, E. Buslaeva et al.· Protein Expression and Purif...· 0 citations
This review summarizes recent advances in engineering key expression elements underlying heterologous protein production in K. phaffii, with particular emphasis on promoter architecture redesign, signal peptide replacement and sequence engineering, molecular chaperone co-expression, and quantitative regulation of the u...
Ru-Yue Han, Ruizheng Hu, An-Ran Liu et al.· Journal of Fungi· 1 citation
A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.
Federico Bosetto, Maria Zacharopoulou, M. Bycroft et al.· bioRxiv· 0 citations
A key goal of many bacterial engineering projects is to produce protein, due to their potent chemical and material functions. However, recombinant proteins are usually produced intracellularly, and secreting arbitrary proteins directly into an extracellular environment is challenging. In this work, we explored recomb...
Anton Kan, S. Emani, Neel S. Joshi· ACS Synthetic Biology· 0 citations
Pichia pastoris is a widely used host for recombinant protein production because it combines the advantages of microbial cultivation with eukaryotic protein folding and secretion. However, secretion efficiency is often limited by the folding capacity of the endoplasmic reticulum (ER), where recombinant proteins must...
Protein-glutamine glutaminase (PGG) is a promising enzyme for improving the functionality of plant proteins, but its industrial production is limited by low native yields and the need for proteolytic activation. Here, we developed a standardized modular Golden Gate-based secretion platform in
Bacillus subtilis...
Clàudia Lliso-Pascual, Sergi Abad, Marc Carnicer et al.· Applied Microbiology and Bio...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
