Oct 2026· ACS Food Science & Technology· 0 citations· 113 references
Microbial Metabolic Engineering and Bioproduction
Abstract
Lactic acid bacteria (LAB) are generally recognized as safe, which makes them ideal hosts for producing food-related chemicals. Traditional metabolic engineering has mostly focused on editing one or a few genes, which often fail to meet the demands of industrial production. High-throughput metabolic engineering based on large-scale genome editing tools holds considerable promise for accelerating the construction of industrial strains. Nevertheless, achieving high-throughput metabolic engineering in Laboratories, which requires large-scale systematic modification of entire metabolic networks, remains a major challenge. This Review first summarizes the progress in the metabolic engineering of LAB. It then highlights the application of CRISPR/Cas9 for precise gene editing in the LAB. Next, we systematically review the large-scale genome editing tools in bacteria. Finally, we discuss future directions for developing large-scale genome editing tools in Laboratories. Through these efforts, we expect that LAB can be developed into efficient microbial cell factories for the sustainable production of food chemicals.
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Zero-knowledge proofs can verify computing results without rerunning workloads or exposing sensitive data. Magnetar helps bring them into the mainstream with support for standard programming languages, major speed gains, and quantum-resistant security. The post Proof-based general-purpose computing with Magnetar appeared first on Microsoft Research.
