Generation of high-quality reference control panels of human induced pluripotent stem cell clones from PBMCs.

Abstract

High-quality, standardized iPSC reference lines are crucial for disease modeling and regenerative medicine. We generated and extensively characterized thirteen iPSC clones from PBMCs of five healthy donors (three female, two male) using non-integrating Sendai virus. This resource is distinguished by its standardized derivation and deep characterization and the deliberate generation of multiple clones per donor to capture and control for clonal variation. These validated lines are available to academic and commercial researchers to accelerate advances in stem cell biology and tissue engineering.