Generation of high-quality reference control panels of human induced pluripotent stem cell clones from fibroblasts.

Abstract

Well-characterized iPSC reference panels are critical for developmental and disease-modeling research. We generated eleven iPSC clones from fibroblasts collected from four healthy, unrelated donors (two female, two male) using non-integrating Sendai virus reprogramming. Each clone was extensively validated according to current best practices, demonstrating genetic stability, high expression of markers for the undifferentiated state and robust trilineage differentiation potential. This collection is distinguished not only by the depth of characterization and standardized workflows, but also by the deliberate inclusion of multiple clones per donor to capture and control for clone‑to‑clone variability. All clones are available to academic and commercial researchers.