CRISPR/Cas-Mediated Genome Editing for Developing Herbicide Tolerant Rice: A Step-by-Step Protocol.

D. Sreekanth Chandrakant Singh D. Pawar M. K. Yadav I. K. Sahadeo P. S. Basavaraj Rajeev Kumar Survi Mahesh

2026 · Methods in molecular biology · Vol 3017, pp. 47-57 · 0 citations

Medicine

TL;DR

This protocol presents a step-by-step approach for generating bispyribac sodium-tolerant rice using the CRISPR/Cas-mediated editing of the ALS gene, and offers a robust framework for producing HT rice lines, potentially reducing reliance on conventional herbicide regimes and mitigating the risk of resistance development in weed populations.

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