AgroGem: A Rapid and Scalable Transient Transformation System for Functional Genetics in Multiple Plant Species

Establishment of an efficient Agrobacterium-mediated transformation system and CRISPR/Cas9-mediated genome editing of the OvPDS1 gene in Oxalis vulcanicola ‘Sunset Velvet’

CRISPR–Cas technologies for precision genome editing in plants: advances, applications, and future perspectives

This review provides a comprehensive synthesis of a recent advances in CRISPR–Cas technologies and their strategic applications in crop genetics and hybrid breeding, and showcases how these technologies accelerate hybrid breeding by engineering male sterility systems, fixing heterosis, and generating high-throughput mu...

Virus-induced Genome Editing in Plants: Viral Vector Biology, Germline Access and the Evidence Base for Heritable, Transgene-Free Crop Improvement

A critical review evaluates the strength and limits of the evidence for the claim that virus-derived products are transgene-free, and prioritised research directions are proposed, emphasising standardised reporting of progeny-level outcomes, biocontained vector design, and genotype-spanning validation in elite crop bac...

SAM-Targeted CRISPR-Cas9 RNP Delivery Combined with Leaf Regeneration Enables DNA-Free, Non-Chimeric Genome Editing in ‘Fuji’ Apple

iPB-REG is established as a practical strategy for producing uniform genome-edited fruit trees and provide a valuable platform for DNA-free genetic improvement and functional genomics in clonally propagated perennial crops.

Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement

An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species

A one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time and demonstrates the versatility of the modified system for this industrially important genus.