AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
This chapter provides a practical workflow for the implementation of intensity-based chemigenetic biosensors, centered around the HaloAKAR2.1 scaffold, a biosensor for protein kinase A activity.
Extracellular signal-regulated kinase (ERK) governs many cellular processes, and its spatiotemporal activity dynamics are important for downstream signaling specificity. Existing fluorescent ERK activity biosensors, however, fall short in enabling spectrally multiplexed, spatially resolved, or deep tissue measurements....
A novel surface-mutation-guided engineering strategy combined with iterative rounds of rational mutagenesis to improve key photophysical properties of red fluorescent proteins (RFPs) and provides a framework that may be broadly applicable to other pigment-protein systems such as genetically encoded metal-ion indicators...
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