CRISPR/Cas9-mediated knock-out of abnormal chemosensory jump 6 (acj6) gene impacts Helicoverpa armigera antennal lobe structure

CRISPR/Cas9-Mediated Knockout of the Chorion Gene s36 Impairs Ovarian Development and Abolishes Egg Hatching in the Melon Fly Zeugodacus cucurbitae

Findings identify s36 as required for normal ovarian maturation and egg viability in Z. cucurbitae, and as a candidate target for genetic control of this pest.

CRISPR/Cas9-mediated loss of FERMT3 reveals kindlin-3–independent neutrophil activity in LAD-III

Mutations in FERMT3 cause LAD-III by impairing integrin function in neutrophils. Using CRISPR/Cas9, we generated FERMT3 KO cultured neutrophils with normal in vitro granulopoiesis, transcriptomic profiles, and recapitulated defective zymosan-induced ROS production. Basal adhesion and chemotaxis were reduced, whereas st...

EMERGING APPLICATIONS OF CRISPR–CAS9 BEYOND GENE EDITING

This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.

Conditional dCas12-Mediated Gene Knockdown and Complementation in Chlamydia.

A CRISPR/Cas9 gene-editing platform for the rice leaffolder, Cnaphalocrocis medinalis.

A CRISPR/Cas9 toolkit was established for the rice leaffolder using entirely endogenous regulatory elements and provides a system for functional genomics in this pest.

mTREX2 co-expression promotes large deletions in CRISPR/Cas12a editing of the highly polyploid sugarcane genome

Efficient CRISPR/Cas12a-mediated targeted mutagenesis of the lw1 gene in sugarcane provides a framework for future engineering of AT-rich cis-regulatory elements in the polyploid sugarcane genome.