High-Resolution Temporal Transcriptome and Metabolome Atlas of Vitis davidii Berry Development Provides Insights into Its Unique Metabolite Accumulation Patterns.
Jul 2026· Journal of Agricultural and Food Chemistry· Vol 74, pp. 23183-23199· 0 citations· 57 references
Medicine
TL;DR
This high-resolution multiomics atlas proposes a foundational regulatory framework for V. davidii berry quality and identifies promising genetic targets for targeted grapevine breeding programs.
Abstract
Mature Vitis davidii berries exhibit a unique metabolite profile characterized by exceptionally low malate, relatively lower soluble sugars, and abundant anthocyanin diglucoside accumulation. To elucidate the regulatory basis underlying these distinctive traits, we performed an integrated transcriptomic and metabolomic analysis across 18 developmental stages. This atlas identified veraison as a critical metabolic reprogramming window, marking a significant shift in metabolic flux that is potentially linked to species-specific transcriptional divergences. Using weighted gene coexpression network analysis (WGCNA), we identified candidate hub genes hypothesized to participate in these shifts. Specifically, transient transformation in grapevine callus provided preliminary functional evidence suggesting that the nuclear-localized transcription factor VdNAC17 potentially regulates soluble sugar accumulation, while VdbZIP30 might serve a dual regulatory function coordinating both soluble sugars and anthocyanins. Collectively, this high-resolution multiomics atlas proposes a foundational regulatory framework for V. davidii berry quality and identifies promising genetic targets for targeted grapevine breeding programs.
This study elucidates the metabolic processes and molecular mechanisms governing postharvest sweetness development in kiwiberry and provides a theoretical foundation for breeding high-quality cultivars with enhanced sweetness.
Zhao Liu, Jianyu Song, Yuying Li et al.· The Journal of the Science o...· 0 citations
This study provides a comprehensive multi‑omics resource and a descriptive framework for understanding transcriptional and metabolic dynamics during sesame floral development, and identifies candidate pathways and genes that may serve as targets for future functional validation and molecular breeding.
Qiyuan An, Hongsen Cheng, Huijie Sun et al.· Frontiers in Plant Science· 0 citations
A systems-level view of blueberry fruit development is provided, highlighting coordinated transcriptional changes across multiple biological pathways during ripening and the identification of conserved gene expression patterns and key regulatory candidates offers valuable targets for improving fruit quality and health-promoting traits in blueberry.
Nayla Zalzalah, Mohamad Elian, Julia C Wozny et al.· BMC Plant Biology· 0 citations
Rapeseed is a globally important oil crop cultivated worldwide, but its proteome remains uncharacterized. Here, we established a quantitative atlas of the proteome and phosphoproteome spanning 24 major tissues. Using Orbitrap Astral MS instrument, we quantified 28,217 proteins and 13,455 phosphoproteins (covering over 60,000 phosphorylation sites) during rapeseed vegetative and reproductive stages, representing the most extensive proteomic and phosphoproteomic dataset for rapeseed to date. Tissue-specific protein expression highlights functional specificity with significant variations between transcript and protein levels across tissues. We also present a phytohormone atlas resource, identifying their key role in tissue specificity. We further integrated phytohormone abundance with transcriptomic, proteomic, and phosphorylation-site profiles using weighted gene co-expression network analysis (WGCNA), revealing partially distinct hormone-associated modules across molecular layers. Functional analysis of BnaTT3, a proteome-prioritized seed hormone-associated candidate, supports its role in coordinating seed development and phytohormone homeostasis. These comprehensive multi-omics resources advance our understanding of rapeseed biology and provide a valuable resource for crop improvement.
Results provide a genetic entry point for metabolic engineering and breeding efforts aimed at modulating grain phenolic profiles and indicate that SbmiR397-5p negatively regulates the expression of SbLAC14 in relation to CT biosynthesis, identifying it as a potential target for manipulating CT metabolism in sorghum.
Yan-Nan Shi, Yong-Chao Guo, Jin-Ping Wang et al.· Plants· 0 citations
Findings reveal the molecular mechanism underlying sowing date-mediated seed quality formation and provide a theoretical basis for high-quality sorghum production.
