Outer membrane stiffness gates multidrug tolerance

A periplasmic regulator establishes adaptive impermeability to control carbapenem entry

Outer-membrane permeability is a major determinant of antibiotic susceptibility in Gram-negative bacteria and is generally thought to be controlled through transcriptional regulation of porin expression. Here we identify the small periplasmic protein PtrA as a regulator of OprD-dependent carbapenem permeability in Pseu...

Antibiotic condensates activate magnesium signaling to drive drug tolerance

These findings establish antibiotic-driven phase separation as a previously unrecognized mechanism to encode cellular signaling and identify antibiotic condensates as a distinct functional unit underlying drug tolerance.

Lipidome plasticity and envelope remodeling under environmental stress in Gram-negative bacteria.

Targeting stress-induced lipid remodeling pathways in Gram-negative bacterial cell envelopes represents a promising therapeutic approach that could sensitize resistant pathogens and guide the development of effective adjuvant strategies.

A natural glycolipid exposes the outer membrane interface as a tunable regulator of bacterial surface attachment

Dalberoside reveals the outer membrane interface as a chemically addressable regulator of bacterial surface attachment, and inhibits adhesion by non-disruptive remodeling of the Gram-negative outer membrane interface.

A lipid fenestration-gated membrane depolarization mechanism expands the repertoire of CRISPR-mediated anti-phage defense strategies

This work characterize Chp1, a membrane-embedded CARF effector that assembles as a tetrameric pore harboring autoinhibitory lipid fenestrations that occlude the pore in the inactive state, and uncover a lipid-gated mechanism that expands the repertoire of CRISPR-mediated defense strategies.

Auranofin shows bactericidal activity in Pseudomonas aeruginosa by targeting thiol homeostasis