Key mechanistic steps coordinating ATP-driven MreB polymerization and turnover are established and provide a basis for a complete MreB assembly–disassembly cycle and for further elucidating how MreB dynamics contribute to cell-wall organization.
Alba de San Eustaquio-Campillo, C. Cornilleau, Sana Afensiss et al.· bioRxiv· 0 citations
It is concluded that surface-mediated polymerization can outpace solution-mediated elongation, even at high actin concentrations (>100 μM); the finite time required for profilin dissociation decreases the advantage conferred by surface-associated polymerases, but only in the absence of force.
R. D. Mullins, Jane Kondev, Kristen Skruber· Biophysical Journal· 0 citations
The Arp2/3 (Actin-Related Protein 2/3) complex nucleates branched actin networks, while myosin motors generate force and reorganize actin through ATP-dependent motor activity. Their coordination is central to membrane remodeling, but the underlying mechanisms differ substantially among cellular processes and organisms....
By directly linking Mg2+-dependent structural compaction to microsecond-scale dynamics, this work reveals fundamental physical principles governing the behavior of recombination intermediates at the single-molecule level.
Chanchal Sharma, A. K. Upadhyaya, D. Sasmal· ChemPhysChem· 0 citations
Actin polymerization into curved and ring‐shaped structures is an evolutionarily conserved mechanism that cells use to generate force, transmit tension, and maintain membrane architecture. In neurons, super‐resolution imaging has revealed a striking periodic submembranous scaffold in axons, composed of circumferential...
A. R. Costa, Luís P. Rodrigues, Monica M. Sousa· Journal of Neurochemistry· 0 citations
The findings uncover a novel product-inhibition mechanism that limits INF2 function and offer important insight into disease mechanisms linked to actin dysregulation.
Felix Uecker, Sven Wargenau, Micaela Boiero Sanders et al.· bioRxiv· 0 citations
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