Regulation of RNA transcript elongation in metazoans and its relevance to disease.

Transcription elongation by RNA polymerase II: from regulatory complexity to disease.

Transcription elongation by RNA polymerase II (Pol II) was originally considered as the monotonic addition of ribonucleoside triphosphates to the growing nascent RNA chain. However, multiple lines of evidence now indicate that transcription elongation is a regulatory hub in gene expression that requires an increasing n...

The molecular basis of transcription initiation by RNA polymerase II.

A dual-module ECAS complex couples transcription elongation and RNA processing.

Faithful gene expression requires coordination between RNA polymerase II elongation and co-transcriptional RNA processing, yet the chromatin-associated assemblies supporting this coupling remain incompletely defined. Using native chromatin proteomics in human cells, we identify ECAS (elongation complex associated with...

Mechanisms of transcription termination across the coding and noncoding loci of the genome.

16S ribosomal RNA modification drives transcript-specific translation efficiency.

It is proposed that MraW modification of 16S rRNA enhances translation efficiency in general, and that specific transcripts have evolved structural features that fine-tune protein levels that may be prevalent in bacteria which exhibit uncoupled transcription and translation.

Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster

Structural studies of eukaryotic RNA polymerase II (Pol II) transcription often rely on in vitro assembly, which may not fully represent native conditions. To investigate Pol II transcription in metazoan cells, we developed a method to isolate native transcription complexes from Drosophila melanogaster embryos using FL...