Adaptive Evolution in Aminoacyl-tRNA Synthetases Drives Antibiotic Tolerance and Resistance in Clinical Klebsiella pneumoniae Isolates.

Mapping Essential Genes in Klebsiella pneumoniae Using Transposon-Insertion Sequencing (Tn-seq)

Klebsiella pneumoniae is a common cause of sepsis in adults and children. Given the emergence of multidrug-resistant isolates, there is an urgent need to develop novel therapeutic strategies. Essential bacterial genes and their products, which are critical to survival and replication, represent intrinsic vulnerab...

Transposon mutagenesis uncovers the genetic landscape of streptomycin susceptibility and implicates SbmA in aminoglycoside uptake in Escherichia coli

Broad adaptations of plasmid-carrying Escherichia coli and Klebsiella pneumoniae to water as a reservoir

Findings demonstrate strain-specific adaptive responses to aquatic environments and underline the context-dependent influence of plasmids in shaping adaptation.

Genomic and structural analysis of dacB variants associated with cephalosporin resistance in Pseudomonas aeruginosa

Structural insight is offered of dacB variants leading to antibiotic resistance and the need to prioritize specific antibiotics to address the challenges arising from protein mutations is emphasized.

Emergence of mutHV76G in carbapenem-resistant Klebsiella pneumoniae disrupts DNA mismatch repair and results in a hypermutator phenotype

ABSTRACT Hypermutation-driven evolution is a major contributor to antibiotic resistance in some bacterial pathogens, but its role in Klebsiella pneumoniae remains poorly defined. We analyzed 11 KPC-producing ST258 K. pneumoniae isolates collected serially over ~4 years from a host with persistent colonization and recur...

Global lessons from antibiotic resistance: metformin-hydrolyzing genes in transposable elements, a new threat for type II diabetic patients?

Metformin pollution appears to promote the emergence and mobilization of pharmaceutical-degrading genes through mechanisms paralleling antibiotic resistance evolution, and these findings reveal a dynamic modular assembly of metabolic functions facilitating adaptation to pharmaceutical pollutants.