RNA-Binding Proteins MCPIP3 and IGF2BP3 Antagonize Lung Cancer Metastasis by Reversibly Regulating METAP2 Expression via mRNA Stability
TL;DR
It is revealed that the RBPs MCPIP3 (monocyte chemotactic protein-induced protein 3) and IGF2BP3 (insulin-like growth factor 2 mRNA-binding protein 3) negatively and positively regulate lung cancer cell metastasis, respectively, by antagonistically regulating METAP2 (methionine aminopeptidase 2) mRNA stability and expression through targeting the same stem–loop structure.
Abstract
Simple Summary Tumor metastasis is one of the key causes of cancer-related deaths, including lung cancer. However, the mechanism controlling the expression of metastasis-related genes has still not been completely clarified, especially the post-transcriptional regulation mechanism mediated by RNA-binding proteins (RBPs) in human lung cancer cells. Here, we revealed that the RBPs MCPIP3 (monocyte chemotactic protein-induced protein 3) and IGF2BP3 (insulin-like growth factor 2 mRNA-binding protein 3) negatively and positively regulate lung cancer cell metastasis, respectively, by antagonistically regulating METAP2 (methionine aminopeptidase 2) mRNA stability and expression through targeting the same stem–loop structure. These findings provide new insights into the yin–yang effects of RBPs in mediating metastasis-related gene expression in human lung cancer cells and may provide potential molecular targets for lung cancer treatment.