free cloning for high throughput gene cloning and expression

A simple multi-cloning strategy for gene expression and subcellular localization in Mycobacteria.

A streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeric fluorescent protein under the control of two promoters of choice is described, providing a fast and flexible cloning strategy for protein expression and subcellular localization analyses in mycobacteria.

A Restriction-Free Cloning Approach for Molecular Engineering of Plasmids.

Simplifying multiplex genome engineering in Saccharomyces cerevisiae with intron-mediated Random Assembly and INtegration (RAIN)

A nascent method for multiplex genome engineering in Saccharomyces cerevisiae that negates the requirement for cloning of expression cassettes is presented and the first reported use of intron-mediated assembly of promoters and ORFs for expression of a functional gene product is reported.

A dual-plasmid system for efficient soluble protein expression in Escherichia coli

Escherichia coli -based expression systems are extensively utilized for recombinant protein production due to their simplicity, cost-effectiveness, and scalability. However, they often face challenges including leaky expression, codon bias, and poor solubility. Various strategies have been developed to address t...

An Efficient Colony PCR Method for Construct Verification in the Basidiomycete Ustilago maydis.

Construction of a versatile cloning vector for Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using benomyl for selection.

Beauveria bassiana is a filamentous fungus that has considerable potential to combat insect pests. Therefore, an easy to use genetic transformation tool helps to investigate which fungal genes play a role in virulence and host specificity. However, B. bassiana is often very resistant to antibiotics used as selective ag...