Skip to content
Log in
Sponsored AI AM CLAUDE, A Beginner's Guide: Learn the basics of working with AI AI AM CLAUDE, A Beginner's Guide: Learn the basics of working with AI
#gene editing Open access

Efficient Microinjection and CRISPR/Cas9 Mediated Genome Editing in Urechis unicinctus

Kai Wang Jiao Chen Shumiao Bai Qing Wang Wei Zhang Zhen-Kui Qin Zheng-Rui Zhang Zhifeng Zhang Yu-Bin Ma
Sep 2026 · Marine Biotechnology · Vol 28 · 0 citations · 64 references
Medicine

TL;DR

A CRISPR/Cas9 genome editing workflow is established for U. unicinctus based on an optimized microinjection procedure, providing a technical framework for functional genomics in echiurans and facilitating future studies of developmental mechanisms and molecular breeding in this species.

DOI PubMed Semantic Scholar
Read PDF

Similar papers

Open access Aug 2026

Endogenous gene tagging in the model brown alga Ectocarpus using a simplified CRISPR/Cas method

A comprehensive CRISPR-Cas genome-engineering framework for Ectocarpus is established that enables both targeted gene disruption and precise genomic insertion and establishes Ectocarpus as a genetically tractable system for functional genomics, providing a foundation for genome engineering across stramenopiles.

Alexandre Paix, Morgane Raphalen, Elena Avdievich et al. · 0 citations
Open access Sep 2026

Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing of Schistosoma mansoni eggs as key to success

Lack of transgenesis methods impedes functional studies in platyhelminths. We present a substantially improved ribonucleic protein complex (RNP)–based genome editing approach for Schistosoma mansoni comparing Cas9 and Cas12a, the latter attained more efficient homology-directed insertion of a 5′-C6-PEG10–modified doubl...

Max F. Moescheid, Prapakorn Wisitpongpun, Vanessa Gross et al. · 1 citation
#gene editing Sep 2026

CRISPR-Cas9 Genome Editing in Mesorhizobium ciceri Through nodC Disruption for Chickpea Symbiosis Studies.

A streamlined genome-editing strategy using a broad-host-range Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (Cas9) system adapted for M. ciceri is described, providing an efficient and reproducible framework for precise gene disruption and functional genomics studies in Mesorh...

Nandita Pasari, Nishant Bhandhari, Shruti et al. · 0 citations
#gene editing Sep 2026

Efficient scarless gene editing in Pichia pastoris via survival stress-based intramolecular homologous recombination.

Nearly 100% knockout efficiency and over 86.67% integration efficiency were achieved in the described experimental cases with this novel pop-in/pop-out-based editing system, which provides a new gene-editing tool for synthetic biology in Pichia pastoris.

Guo-Wei Zhao, Liang-Cheng Jiao, Yun-Chong Li et al. · 0 citations

Related blog posts

Microsoft Research Blog Oct 15, 2026

Proof-based general-purpose computing with Magnetar

Zero-knowledge proofs can verify computing results without rerunning workloads or exposing sensitive data. Magnetar helps bring them into the mainstream with support for standard programming languages, major speed gains, and quantum-resistant security. The post Proof-based general-purpose computing with Magnetar appeared first on Microsoft Research.

We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.